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简述原种接种操作过程

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正确答案:

(1)手、母种管、接种工具酒精消毒,点燃酒精灯。本题解析:试题答案(1)手、母种管、接种工具酒精消毒,点燃酒精灯。

(2)左手持母种管、右手持接种镐并灼烧(竖直、横拉)。

(3)拔掉棉塞,灼烧管口,并以火焰封住管口。

(4)将接种铲伸进母种管内,待冷凉,弃除前端1cm处的培养基,接种镐一经灭菌后不许接触未经灭菌的物体。

(5)将母种菌种切成5段,迅速移进原种瓶口,菌种不能掉外面(同时另一人将瓶口打开,并以火焰飞封口)。

(6)要以原种瓶口迎母种管口,不能反向。

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