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请叙述三级菌种培养基的制备过程

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正确答案:

木腐生菌类:木屑三级菌种制备方法与木屑二级种几乎相同,所不同的仅是在洗涤前菌种瓶的正中央打一锥形孔,孔径2cm。打孔的目的是接种时,菌种能从预留孔落入,便于菌种瓶内培养基表面和空穴内菌丝同步蔓延,缩短培育时间,有利于容器内菌丝的菌龄基本相同。

草腐生菌类:

1.麦粒三级菌种的制作:

1).浸泡,清洗,去尘土

2).摊晾、拌料

3).装瓶

4).洗瓶,塞棉花

5).灭菌、冷却

2.稻草段三级菌种的制作:取无霉烂,新鲜的干稻草段切成3-4cm长,浸泡3-5小时后沥干,撒入麸皮,拌入石膏,过磷酸钙,即可装瓶,压瓶,洗涤,塞棉花,立即灭菌。

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